Most of the conventional methods for the analysis of specific dna sequences are based on the nucleic acid hybridization 目前,它已廣泛應(yīng)用在生命科學(xué),尤其是醫(yī)學(xué)的各個領(lǐng)域。
Experiments of nucleic acid hybridization show that taqman 6 only hybridizes to the pcr products amplified from s . costatum , but not to products from other microalga . these results indicate that primer6 ( f / r ) and taqman6 can be used to establish the rfq - pcr method for the quantitative detection of s . costatum 最后以primer6rdna序列在幾種浮游植物的分類及中肋骨條藻定量檢測中的應(yīng)用( f / r )和taqman6探針建立了定量檢測中肋骨條藻的rfq一pcr 。
Section two the progresses in detections of staphylococcus aureus and staphylococcal enterotoxins in this section , the author introduced classification , typology and toxicological study of staphylococcus aureus in addition to relevant determination methods including pcr technique , nucleic acid hybridization technique , biosensor technique , superantigen technique 館: researchonp滋oeleetr紀(jì)biosensorsforlhedetectionofd入月encodingstaphyloeocealenterotoxinb第二章s戌w壓電生物傳感器測定b型葡萄球菌dna的研究本章建立了一種用于b型葡萄球菌腸毒素檢測的saw壓電生物傳感器。
Therefore , the determination of seb is very important for food hygienic analysis as well as for clinical analysis . nucleic acid hybridization technique is one of the widely - used methods in molecular biology and gene technology . the present work has developed piezoelectric biosensors used in the detection of seb dna by tacking the piezoelectric quarts crystal as a sensitive component while synthetic oligonucleotide probe as recognize molecule 其中b型葡萄球菌腸毒素( seb )是一種通常條件下更穩(wěn)定,毒性最強(qiáng)的毒素,而核酸雜交技術(shù)則是分子生物學(xué)和基因工程中最常用和最基本的方法之一,因此本論文以該毒素的產(chǎn)毒基因?yàn)闄z測對象,以壓電石英晶體為敏感元件,以合成的寡核苷酸探針為識別分子,構(gòu)建了用于seb基因檢測的壓電生物傳感器。